The biology laboratory is a 50 m2 facility which serves to prepare biological and biophysical samples for measurements at the neutron scattering instruments. It is equipped to pre-characterise samples by UV-VIS spectroscopy and dynamic light scattering. A low-temperature freezer can be used to store samples at temperatures down to -80°C.
Booking of instruments
After signing up for a GhOST-Account you can use our booking site to view and reserve our instruments for usage. If you have any questions feel free to contact us (contact information on the right).
Equipment
Consumables
- Syringe needles and syringes (1 – 10 ml)
- Pipette tips for 1 µl to 5 ml
- Piston pipettes (last maintenance in July 2017; with certificate)
- Nitrile gloves in sizes S to XL
- “Solvent cabinet in the chemistry laboratory (it is best to send an e-mail in advance so that we can check whether the required chemical is available)”:/media/img_0357.jpg
- 20 ml Vials Perkin Elmer with cap
- Eppis 0.5; 1; 2 ml
- Greiner tubes 15 and 50 ml
- Measuring pipettes made of glass and plastic
- Volumetric pipettes
- Deuteriumoxide
- Well plates
- Glass (Schott bottles, beakers, watch glasses etc.)
- Kimtech wipes
- etc.
If you have any questions about the consumables, please contact us (contact information on the right).
FT-IR Spectrophotometer Perkin Elmer Spectrum Two with DTGS detector
Picture of the instrument
Specifications:
- Dimensions(W x D x H): 450mm x 300mm x 210mm
- Weight: approx. 13kg
- Measuring range: 350cm -1 – 8300cm -1
- Accessories: Universal ATR Sampling Accessory
Stereo Microscope Olympus SZX7
Picture of the instrument
Screenshot of the software
Accessories & specifications:
- Zoom: 0.8x – 5.6× (7 Steps)
- Objective: Olympus DF PLAPO 1 X -4
- Camera: Olympus UC90
- Aperture: Olympus SZX2-AN
Lighting:
- Upper: Schott EasyLED R+:
- Lower: Olympus KL 1600 LED
Ocular:
Olympus WHSZ10X-H:
- Zoom: 10x (total: 8×-56x)
- Dioptrin range: -8 – +5
- Visual field number: 22
Particlsizer AntonPaar Litesizer 500
Picture of the instrument
Specifications:
- Dimensions(W x D x H): 460mm x 485mm x 135mm
- Weight: 18kg
- Laser: 658nm, 40mW power
- Measurement particle size: 0.3nm- 10µm
- Measurement zeta potential: 3.8nm – 100µm
- Min. concentration(Measurement particle size): 0.1mg/mL
- Min. concentration(Measurement zeta potential): 1mg/mL
- Temperature: 0℃ -90℃
- possible environmental setting with pH Titration system by Metrohm
Nanophotometer Implen NP80
Picture of the instrument
Specifications:
- Wavelength range: 200nm – 900nm
- Wavelength accuracy: +/- 0.75nm
- Absorption deviation(meas. at 0.7A): <1.75%
- Dimensions(B x T x H): 200mm x 200mm x 140mm
- Equipped with Touchscreen
pH Titration System Metrohm
Picture of the System
Components:
Metrohm 867 pH module:
- Measurement range voltage: 0mV – 1200mV
- Measurement accuracy voltage: +/- 0,2mV
- Dimensions(W x D xH): 142mm x 108mm x 230mm
- Weight: 2660g
Metrohm 801 Stirrer
- Max. rotating speed: 1700 – 1900 U/min
- Dimensions(W x D x H): 90mm x 430mm x 140mm
- Weight: 613g
Metrohm 800 Dosino(3mal)
- Dimensions(W x D x H): 98mm x 67mm x 83mm
- Weight: approx. 410g
Bench-top Incubator Molecular Dimensions
Picture of the instrument
Specifications:
- Used for protein crystallisation
- Temperature: 4℃ – 70℃ (Lowest temperature a maximum of 16℃ beneath roomtemperature, therefore approx. 10℃ in our lab)
- Temperature accuracy: +/- 0.5℃
- Thermostat accuracy: +/- 0.2℃
CD Spectrophotometer Jasco J1100
Picture of the instrument
Specifications:
- Wavelength range: 180 – 600nm
- Wavelength accuracy(180-250nm): +/-0.2nm
- Wavelength accuracy(250-500nm): +/-0.4nm
- Wavelength accuracy(500-600nm): +/-0.8nm
- Absorption range: 0-5
- Absorption accuracy: +/- 0.01
UV-Vis Spectrophotometer Jasco V770
Picture of the instrument
Screenshot of the software
Short instructions
Specifications:
- Wavelength range: 190 – 3200nm
- Wavelength accuracy(656.1nm): +/- 0.3nm
- Wavelength accuracy(1,312.2nm): +/- 1.5nm
- Absorption(UV& visible): 0 – 3
- Absorption(NIR): 0 – 3
- Absorption accuracy(0-0.5): +/- 0.0015
- Absorption accuracy(0.5-1): +/- 0.0025
Accessories:
Drying Cabinet Memmert UNB 300
Picture of the instrument
Description:
The drying cabinet has an easy-to-clean stainless steel working chamber reinforced with deep-drawn ribbing with protected integrated 4-sided all-round heating and a stainless steel grate. It is locked via a fully insulated stainless steel door with double locking and 4-point adjustment as well as a fully galvanized sheet steel rear wall.
The temperature is controlled by heating and ventilation, so temperatures below room temperature cannot be achieved. If the heating element sensor fails, the heating is permanently switched off approx. 10°C above the setpoint. If the switching element is defective, an additional relay takes over the continuation of the heating control approx. 10°C above the setpoint. If the sensor is faulty (incorrect measured values) or if the controller fails completely, the temperature limiter (TB protection class 1) switches off the heating approx. 10°C above the maximum cabinet temperature (220°C).
Specifications:
- Adjustable temperature: 20°C – 220°C (with overheating protection)
- Display: digital LED display for current and target temperature as well as remaining runtime
- Display resolution: current and target temperature display 0.5 °C
- Runtime setting: integrated digital timer (1 min to 99 h 59 min)
- Control unit: micro processor- PID-temperature controller with integrated self-diagnose system
- Ventilation: exhaust air connection with throttle damper (room air)
- Dimensions(outside)(W x D x H): 630mm x 600mm x 400mm
- Dimensions(inside)(W x D x H): 480mm x 320mm x 250mm ; 38.4 l
- Power supply: 230V at 5.3 A; 50/60 Hz
- Power: 1200 W (heating)
- Weight: approx. 32 kg
- Material: stainless steel
Short manual:
- Switch the power by pressing the on/off latching button
- Hold down “Set”-button and adjust the target temperature by turning the matching button
- Adjust the ventilation with the stemless controller
UV-Light Hamamatsu LC8 L9588
Picture of the instrument
Specifications:
- Lightsource: 200 W Quecksilber-Xenon Light
- UV intensity (typ.): 4500 mW/cm2 typ. (at 365 nm, -01 A Typ)
- Wavelength: adjustable via light filter (shown on instrument)
- Running temperature: 5 – 35 °C
- Dimensions(W x D x H): 142mm x 227mm x 360mm
- Power supply: 100 V AC – 240 V AC (Auto-adjusting) 47 – 63 Hz
- Weight: approx. 6.4 kg
Bench-top centrifuge Sigma 3K30
Picture of the instrument
Specification:
- Rotational Speed: 100 – 30,000 rpm (100 rpm steps)
- Max. RCF: 64,397 x g
- Rotor capacity(24 × 1.5 – 2.2ml):* max. 26,000 rpm
- Rotor capacity(10 × 10ml): max. 26,000 rpm
- Rotor capacity(6 × 30ml): max. 26,200 rpm
- Rotor capacity(6 × 50ml): max. 21,000 rpm
- Rotor capacity(4 × 85ml): max. 20,000 rpm
- Rotor capacity(4 × 100ml): max. 5,000 rpm
- Acceleration time: 90s
- Acceleration time: 58s
- Timer: 15s – 599min
- Adjustable temperature range: -20ºC – 40°C (1 °C steps)
- Noise emission: <64dB(A)
- Power supply: 230V, 50Hz
- Max. Power usage: 1260W
- Dimensions(W x D x H): 550mm x 650mm x 400mm
- Weight: approx. 100kg
FPLC System GE Healthcare ÄKTA
Picture of the system
Screenshot of the software
Short instructions
Details:
- Used for protein purification
- Supported by Unicorn 5.11 Software
Specifications of the parts:
Pump: P-920:
- Flowrate (isocratic): 0.05–20 ml/min in 10 µl/min steps
- Flowrate (gradient): 0.1–20 ml/min in 10 µl/min steps
- Pressure range: 0–5 MPa (50 bar, 725 psi)
- Pulse pressure: Max. 6% (dP/P) while pumping
- Stable pH range: 1-13 (1-14 under 1 day of continuous usage)
- Viscosity < 10 ml/min: Max. 10 cP
- Viscosity > 10 ml/min: Max. 5 cP
- Repeatability of the flow rate (0.5-10 ml/min): rsd < 0.2%
- Repeatability of the flow rate (10-20 ml/min): rsd < 0.5%
- Mixing ratio accuracy (between switches): ± 2% at 0.5–5 ml/min and < 5 MPa
- Mixing ratio accuracy(during switches): ± 2% at 0.5–5 ml/min and 0.5–2.0 MPa
- Repeatability of the mixing ratio: rsd < 0.5% st 0.5–20 ml/min and < 5 MPa
Spectrophotometer: Monitor UPC-900:
- Absorption range: 0.01-5.0 AU (ganze Skala)
- „Autozero“ range: -0.2-2.0 AU
- Baseline setting: adjustable from 0 to 100% of the whole scale
- Wavelength Hg light (unadjustable): 254 and 280nm
- Wavelength (adjustable by filter): 313, 365, 405, 436 and 546nm
- Wavelength Zn light: 214 nm
- Path length in the flow cell: 5mm
- Volume of the flow cell: 6 µl (10 µl detector volume)
- Flowrate: 0-20 ml/min
- Max. pressure: 4.0 MPa (40 bar, 580 psi)
- Max. back pressure: 0.02 MPa bei 20 ml/min
- Flowrate of the flow cell for measurement of the conductivity: 0-100 ml/min
- Conductivity measuring range: 1 µS/cm – 999.9 mS/cm
- Max. pressure (conductivity measuring): 5 MPa (50 bar, 725 psi)
- Max. back pressure (conductivity measuring): 0.01 MPa at 100 ml/min
Fractionalization: Frac-920
Mixer: M-925
Valve: INV-907
Cold light source Schott KL 2500 LCD
Picture of the instrument
Specifications:
- Total luminous flow at the light output(level4): 500 lm
- Total luminous flow at the light output(level5): 1000 lm
- Total luminous flow at the light output(level6): 1300 lm
- Light entry angle(2αeff): 53
- Color temperature: ca. 2000 K bis 3400 K
- Useable bundle diameter of the light guide: max. 15 mm
- Max. filter in the filter wheel: 5 filter
- Halogen reflector lights: Osram HLX 64653/ Philips 13163
- Dimensions(W x D x H): 200 mm x 265 mm x 170 mm
- Weight: 6 kg
pH/Ion Measuring Instrument Metrohm 913
Picture of the instrument
Specifications:
- pH-resolution: 0.001/0.01/0.1 (adjustable)
- pH relative accuracy: ± 0.003 pH
- Storage: 10000 measuring points in RAM
- pH calibration: Up to 5 points out of 7 predefined buffers
- Isopotential point: pH 7.00
- Outputs: USB A/B 2.0USB (Data transfer); USB-Y (Printing)
- pH input: BNC; Impendance >3 * 1012 Ω
- Temperature input: NTC 30 kΩ, Pt1000
- Dimensions(W x D x H): 204 mm x 174 mm x 74 mm
- Weight: 890 g
Available electrodes:
Biotrode:
- Usage: pH-measuring in small Volumina
- Electrolyte leakage: 3-30µl/h
- Min. immersion depth: 7mm
- Measurement range: 1pH-11pH
- Membran-resistance: 300-600
- Resistance: 30kΩ
- Temperature: 0ºC-60ºC
Mikroelectrode / 125mm:
- Usage: Routine use on the sample changer & in smaller measuring vessels for acid/base titrations
- Electrolyte leakage: 5-15µl/h
- Min. Immersion depth: 20mm
- Measurement range: 0pH-14pH
- Membran-resistance: 200-500
- Resistance: 5kΩ
- Temperature: 0ºC-80ºC
Universal Measurement Instrument Ahlborn ALMEMO 2490-2
Picture of the instrument
Specifications:
- Operating temperature: -10ºC bis +50°C
- Storage: 99 measurements in the RAM
- Sensor voltage supply: 9V, max. 150mA
Measurement sensors:
Dew point sensor
Contact
Dr. Tobias Schrader
Phone: +49 (0)89 158860-743
E-mail: t.schrader@fz-juelich.de
Laboratory technicians:
Tabea Bartelt
Phone: +49 (0)89 158860-733
E-mail: t.bartelt@fz-juelich.de
Milena Jerosch
Phone: +49 (0)89 158860-765
E-mail: m.jerosch@fz-juelich.de
Laboratory: Building UYL, Room 03.27
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