MLZ is a cooperation between:

Technische Universität München> Technische Universität MünchenHelmholtz-Zentrum Geesthacht> Helmholtz-Zentrum GeesthachtForschungszentrum Jülich> Forschungszentrum Jülich
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MLZ (eng)

Lichtenbergstr.1
85748 Garching

Cryo-Transmission Electron Microscopy

Recently a new transmission electron microscope (TEM) was installed at the MLZ in the Neutron Guide Hall East building (UYM, Room 3.28).
Transmission electron microscopy is a complementary technique with neutron scattering in the frame of soft matter studies. With the TEM, real space investigations are performed to access information about shape, size and size distribution of particles, self-assembly and aggregation. Users will be supported by JCNS scientists (M.S. Appavou) to conduct the suitable preparation and TEM investigation.

Specifications:

  • JEOL 200 kV JEM-FS2200 with a field emission gun (FEG).
  • Magnification from 50x to 1 500 000x, resolution of 0,2 nm in point and 0,1 nm in lattice.
  • Tietz CCD camera with 2048 × 2048 pixels.
  • Grid preparation by carbon sputtering on bare grids.
  • Glow discharge process to make the coated grids hydrophilic.
  • Plunge freezer to prepare cryo-specimen for direct imaging (Cryo-DI), for freeze fracture and direct imaging (FFDI) or freeze fracture before shadowing, Pt/C or Ta/W, so-called freeze fracture and electron microscopy (FFEM).
  • Cryo-specimen investigation at low temperature around -180°C under vacuum.
  • Ultramicrotome for room temperature and cryo sectioning.

New offer:

The JCNS TEM laboratory is open for users to complement neutron experiments at the MLZ

The objective of the JCNS Transmission Electron Microscopy (TEM) laboratory is to offer the preparation and investigation of specimen to users who wish to complement their neutron experiments at the MLZ by using TEM either on-site or remotely.

TEM beamline application must be submitted jointly with the related neutron scattering beamtime proposal

Prior to proposal submission it is mandatory for the user to contact the TEM laboratory scientist in order to discuss the feasibility of the research idea and the required time for the TEM investigations.

The terms of the use of the TEM facility are described in the following concept document:

TEM user concept

TEM beamtime application has to be done by filling the template for download:

Please inquire further information about the application procedure from Dr. M.-S. Appavou (see contact).

Contact:

Dr. Marie-Sousai Appavou
Phone: +49 (0)89 289-10747
E-mail:

Lab location: Building UYM (Neutron guide hall East, upper level), room 03.28

JEM-FS2200 Transmission electron microscope at the JCNS@FRM2
JEM-FS2200 Transmission electron microscope at the JCNS@FRM2

JEM-FS2200 Transmission electron microscope at the JCNS@FRM2

High Quality Ultramicrotome for Precise Room Temperature and Cryo Sectioning
High Quality Ultramicrotome for Precise Room Temperature and Cryo Sectioning

High Quality Ultramicrotome for Precise Room Temperature and Cryo Sectioning

Docking station and Carbon Sputtering Coater
Docking station and Carbon Sputtering Coater

Docking station and Carbon Sputtering Coater

Freeze Fracture and Etching System
Freeze Fracture and Etching System

Freeze Fracture and Etching System

Automatic Plunge Freezer
Automatic Plunge Freezer

Automatic Plunge Freezer

Application example:

TEM_Example

Magnetite particles embedded in gelatin matrix

MLZ is a cooperation between:

Technische Universität München> Technische Universität MünchenHelmholtz-Zentrum Geesthacht> Helmholtz-Zentrum GeesthachtForschungszentrum Jülich> Forschungszentrum Jülich