MLZ is a cooperation between:

Technische Universität München> Technische Universität MünchenHelmholtz-Zentrum Geesthacht> Helmholtz-Zentrum GeesthachtForschungszentrum Jülich> Forschungszentrum Jülich
Logo

MLZ

Lichtenbergstr.1
85748 Garching

» zurück

Dynamics of Proteins and Photosynthetic Membranes explored by Neutron Spin Echo Spectroscopy

Large-scale domain dynamics in proteins are found when flexible linkers or hinges connect protein domains. As larger domains contribute to the conformational changes, the timescale of the involved motions is slowed down. The role of slow domain dynamics is being increasingly recognized as essential in understanding the function of proteins like rearranging the active center after substrate binding, or allowing transport and release of various products. Neutron Spin-Echo (NSE) is a high-resolution spectroscopic technique that has proved over the past years to be a successful method of studying the slow dynamics of bio-materials. The NSE technique allows simultaneous observation of correlation times from several picoseconds up to several hundreds of nanoseconds, at correlation length scales relevant for protein domain movements of several nanometers distance between domains. NSE can detect variations in conformational geometry and modes of motion such as: undulation, bending, stretching, effective mass diffusion, rotational and translational diffusion, as well as the presence of protein inter-domains motions, which are not possible using purely energy-resolved spectroscopic techniques. This presentation covers the basic principles of NSE and two applications of NSE on domain dynamics of Immunoglobulin G1 antibody and on flexibility of cyanobacterial thylakoid membrane sheets in living cyanobacterial cells.

Seminar: Neutronen in Forschung und Industrie

Datum12.06.2017
Uhrzeit14:30 - 15:30 Uhr
OrtGarching, Germany
RaumHS3, Physik Department
SprecherDr. Laura-Roxana Stingaciu, SNS
Veranstalter

MLZ, TUM

MLZ is a cooperation between:

Technische Universität München> Technische Universität MünchenHelmholtz-Zentrum Geesthacht> Helmholtz-Zentrum GeesthachtForschungszentrum Jülich> Forschungszentrum Jülich